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Candida albicans library (LMBP LIB21)
| Library name: | Candida albicans library |
| Library type: | gDNA |
| Library accession number: | LMBP LIB21 |
| Organism: | Candida albicans |
| Vector name: | YCp50 |
| Vector sequence file: | ycp50.txt |
| Vector circular map: | ycp50.pdf |
| Vector accession number: | ATCC 37419 |
| Vector type: | yeast expression vector |
| Cloning sites: | BamHI |
| Library coverage: | 1,5 x 10E5 |
| Average insert size: | 10-23 kb |
| Distributed as: | pooled library DNA |
| DNA concentration: | 800 ng/µl |
| Volume: | 5 µl |
| Further information: | YCp50 was BamHI cut and the genomic Candida albicans Sau3AI fragments were ligated. The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones. |
| History of deposit: | The gDNA library was deposited by Janssen Pharmaceutica N.V.1. 1 Beerse, Belgium |
| Library reference: | De Backer et al., Nat. Biotechnol. 19 (2001), 235-241 [PMID: 11231556] |
| Related reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
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